PDBsum entry 8hyh

Oxidoreductase

PDB id: 8hyh

Contents

Protein chain

366 a.a.

Ligands

NAD

EDO ×2

PYR

Waters ×163

PDB id:

8hyh

Name:

Oxidoreductase

Title:

Structure of amino acid dehydrogenase3448

Structure:

Alanine dehydrogenase. Chain: a. Engineered: yes

Source:

Geobacillus kaustophilus. Organism_taxid: 1462. Gene: gk3448. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.39Å R-factor: 0.185 R-free: 0.211

Authors:

H.Sakuraba,T.Ohshima

Key ref:

M.Maeno et al. (2023). Two different alanine dehydrogenases from geobacillus kaustophilus: their biochemical characteristics and differential expression in vegetative cells and spore. Biochim biophys acta, 1871, 40904. PubMed id: 36918121

Date:

06-Jan-23 Release date: 05-Apr-23

Protein chain

Q5KUA3  (Q5KUA3_GEOKA) -  Alanine dehydrogenase from Geobacillus kaustophilus (strain HTA426)

Seq: 377 a.a.

Struc: 366 a.a.

Enzyme reactions

• Enzyme class: E.C.1.4.1.1 - alanine dehydrogenase.
• Reaction: L-alanine + NAD⁺ + H2O = pyruvate + NH4⁺ + NADH + H⁺

L-alanine + NAD(+) + H2O (Bound ligand (Het Group name = NAD) corresponds exactly) = pyruvate + NH4(+) + NADH + H(+) (Bound ligand (Het Group name = PYR) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site