PDBsum entry 8htw

Hydrolase

PDB id: 8htw

Contents

Protein chains

179 a.a.

Waters ×62

PDB id:

8htw

Name:

Hydrolase

Title:

Crystal structure of the ring nuclease sso2081 y133f mutant from saccharolobus solfataricus in its apo form

Structure:

Crispr system ring nuclease sso2081. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Saccharolobus solfataricus p2. Organism_taxid: 273057. Strain: atcc 35092 / dsm 1617 / jcm 11322 / p2. Gene: sso2081. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta

Resolution:

2.00Å R-factor: 0.224 R-free: 0.256

Authors:

Z.Lin,L.Du,Z.Luo

Key ref:

L.Du et al. Molecular basis of stepwise cyclic tetra-Adenylate cl by the type III crispr ring nuclease crn1/sso2081. Nucleic acids res, . PubMed id: 36807980

Date:

21-Dec-22 Release date: 15-Feb-23

Protein chains

Q7LYJ6  (Q7LYJ6_SULSO) -  CRISPR system ring nuclease SSO2081 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Seq: 178 a.a.

Struc: 179 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.6.1.- - ?????