PDBsum entry 8hsr

Transcription

PDB id: 8hsr

Contents

Protein chains

(+ 0 more) 361 a.a.

229 a.a.

1119 a.a.

1303 a.a.

91 a.a.

DNA/RNA

Ligands

ADP ×6

BEF ×6

Metals

_MG ×7

_ZN ×2

PDB id:

8hsr

Name:

Transcription

Title:

Thermus thermophilus rho-engaged rnap elongation complex (composite structure)

Structure:

Transcription termination factor rho. Chain: a, b, c, d, e, f. Engineered: yes. DNA-directed RNA polymerase subunit alpha. Chain: g, h. Synonym: rnap subunit alpha,RNA polymerase subunit alpha, transcriptase subunit alpha. Engineered: yes. DNA-directed RNA polymerase subunit beta.

Source:

Thermus thermophilus hb8. Organism_taxid: 300852. Gene: rho. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: rpoa, ttha1664. Gene: rpob, ttha1813. Gene: rpoc, ttha1812. Gene: rpoz, ttha1561.

Authors:

Y.Murayama,H.Ehara,S.Sekine

Key ref:

Y.Murayama et al. (2023). Structural basis of the transcription termination fac engagement with transcribing RNA polymerase from ther thermophilus.. Sci adv, 9, e7093. PubMed id: 36753546

Date:

20-Dec-22 Release date: 03-May-23

Protein chains

Q5SJE9  (Q5SJE9_THET8) -  Transcription termination factor Rho from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 426 a.a.

Struc: 361 a.a.

Protein chains

Q5SHR6  (RPOA_THET8) -  DNA-directed RNA polymerase subunit alpha from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 315 a.a.

Struc: 229 a.a.

Protein chain

Q8RQE9  (RPOB_THET8) -  DNA-directed RNA polymerase subunit beta from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 1119 a.a.

Struc: 1119 a.a.

Protein chain

Q8RQE8  (RPOC_THET8) -  DNA-directed RNA polymerase subunit beta' from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 1524 a.a.

Struc: 1303 a.a.

Protein chain

Q8RQE7  (RPOZ_THET8) -  DNA-directed RNA polymerase subunit omega from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 99 a.a.

Struc: 91 a.a.

DNA/RNA chains

G-G-T-G-C-A-C-T-A-C-C-A-C-A-A-G-C-A-C-C-C-A-G-G-T-G-G-A-T-G-T-G-G-A-G-A-T-A 38 bases

U-A-C-C-C-A-U-A-A-C-C-A-U-A-U-C-U-C-C-A-C-A-U-C-C-A-C-C-U 29 bases

T-A-T-C-T-C-C-G-G-G-T-G-C-T-T-G-T-G-G-T-A-G-T-G-C-A-C-C 28 bases

Enzyme reactions

• Enzyme class 2: Chains A, B, C, D, E, F: E.C.3.6.4.- - ?????
• Enzyme class 3: Chains G, H, I, J, K: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site