PDBsum entry 8hrs

Structural protein

PDB id: 8hrs

Contents

Protein chains

335 a.a.

Ligands

NAP ×4

GOL ×3

Waters ×722

PDB id:

8hrs

Name:

Structural protein

Title:

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from corynebacterium glutamicum atcc13032 (l36s/t37k/p192s) in complex with NADP

Structure:

Glyceraldehyde-3-phosphate dehydrogenase. Chain: a, b, c, d. Synonym: gapdh,NAD-dependent glyceraldehyde-3-phosphate dehydrogenase. Engineered: yes. Mutation: yes

Source:

Corynebacterium glutamicum atcc 13032. Organism_taxid: 196627. Gene: gap, cgl1588, cg1791. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.00Å R-factor: 0.173 R-free: 0.227

Authors:

H.F.Son,K.J.Kim

Key ref:

H.F.Son et al. (2023). Structure-Guided protein engineering of glyceraldehyde-3-Phosphate dehydrogenase from corynebacterium glutamicum for dual NAD/NADP cofactor specificity.. J.Agric.Food chem., 71, 17852. PubMed id: 37935620 DOI: 10.1021/ACS.JAFC.3C06176

Date:

15-Dec-22 Release date: 06-Dec-23

Protein chains

Q01651  (G3P_CORGL) -  Glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)

Seq: 334 a.a.

Struc: 335 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.2.1.12 - glyceraldehyde-3-phosphate dehydrogenase (phosphorylating).
• Pathway: Glyceraldehyde-3-phosphate Dehydrogenase (phosphorylating)
• Reaction: D-glyceraldehyde 3-phosphate + phosphate + NAD⁺ = (2R)-3-phospho- glyceroyl phosphate + NADH + H⁺

D-glyceraldehyde 3-phosphate (Bound ligand (Het Group name = GOL) matches with 60.00% similarity) + phosphate (Bound ligand (Het Group name = NAP) matches with 91.67% similarity) + NAD(+) = (2R)-3-phospho- glyceroyl phosphate + NADH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site