PDBsum entry 8hqs

Cell cycle

PDB id: 8hqs

Contents

Protein chains

394 a.a.

408 a.a.

309 a.a.

527 a.a.

322 a.a.

285 a.a.

142 a.a.

PDB id:

8hqs

Name:

Cell cycle

Title:

Cryo-em structure of 8-subunit smc5/6 head region

Structure:

Structural maintenance of chromosomes protein 5. Chain: a. Engineered: yes. Structural maintenance of chromosomes protein 6. Chain: b. Engineered: yes. DNA repair protein kre29. Chain: d. Synonym: killer toxin-resistance protein 29.

Source:

Saccharomyces cerevisiae s288c. Organism_taxid: 559292. Strain: s288c. Gene: smc5. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: smc6. Gene: kre29. Gene: nse5.

Authors:

L.Qian,Z.Jun,Z.Xiang,C.Tong,Z.Wang,J.Duo,C.Zhenguo,L.Wang

Key ref:

L.Qian et al. Cryo-Em structure of 8-Subunit smc5/6 head region. To be published, . PubMed id: 38890552

Date:

14-Dec-22 Release date: 19-Jun-24

Protein chain

Q08204  (SMC5_YEAST) -  Structural maintenance of chromosomes protein 5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1093 a.a.

Struc: 394 a.a.*

Protein chain

Q12749  (SMC6_YEAST) -  Structural maintenance of chromosomes protein 6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1114 a.a.

Struc: 408 a.a.*

Protein chain

P40026  (KRE29_YEAST) -  DNA repair protein KRE29 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 464 a.a.

Struc: 309 a.a.

Protein chain

Q03718  (NSE5_YEAST) -  Non-structural maintenance of chromosome element 5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 556 a.a.

Struc: 527 a.a.

Protein chain

Q07913  (NSE1_YEAST) -  Non-structural maintenance of chromosomes element 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 336 a.a.

Struc: 322 a.a.

Protein chain

Q05541  (NSE3_YEAST) -  Non-structural maintenance of chromosome element 3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 303 a.a.

Struc: 285 a.a.

Protein chain

P43124  (NSE4_YEAST) -  Non-structural maintenance of chromosome element 4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 402 a.a.

Struc: 142 a.a.

* PDB and UniProt seqs differ at 304 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain F: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine