PDBsum entry 8hq6

Transport protein

PDB id: 8hq6

Contents

Protein chains

208 a.a.

129 a.a.

995 a.a.

Ligands

NO3 ×14

GOL ×5

GTP

GLU

DMS ×4

MFF

Metals

_MG

_CL ×2

Waters ×689

PDB id:

8hq6

Name:

Transport protein

Title:

Kl2 in complex with crm1-ran-ranbp1

Structure:

Gtp-binding nuclear protein ran. Chain: a. Synonym: androgen receptor-associated protein 24,gtpase ran,ras-like protein tc4,ras-related nuclear protein. Engineered: yes. Mutation: yes. Yrb1 isoform 1. Chain: b. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ran, ara24, ok/sw-cl.81. Expressed in: escherichia coli. Expression_system_taxid: 562. Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932.

Resolution:

2.03Å R-factor: 0.193 R-free: 0.224

Authors:

Q.Sun,L.Jian

Key ref:

L.Jian et al. (2023). Discovery of aminoratjadone derivatives as potent noncovalent crm1 inhibitors.. J med chem, 66, 11940. PubMed id: 37595020

Date:

13-Dec-22 Release date: 25-Oct-23

Protein chain

P62826  (RAN_HUMAN) -  GTP-binding nuclear protein Ran from Homo sapiens

Seq: 216 a.a.

Struc: 208 a.a.*

Protein chain

P41920  (YRB1_YEAST) -  Ran-specific GTPase-activating protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 201 a.a.

Struc: 129 a.a.

Protein chain

P30822  (XPO1_YEAST) -  Exportin-1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1084 a.a.

Struc: 995 a.a.*

* PDB and UniProt seqs differ at 13 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 3: Chains B, C: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.