PDBsum entry 8hpa

Viral protein

PDB id: 8hpa

Contents

Protein chains

1004 a.a.

426 a.a.

218 a.a.

DNA/RNA

PDB id:

8hpa

Name:

Viral protein

Title:

Monkeypox virus DNA replication holoenzyme f8, a22 and e4 complex in a DNA binding form

Structure:

DNA polymerase. Chain: a. Engineered: yes. DNA polymerase processivity factor component a20. Chain: c. Engineered: yes. E4r. Chain: b. Engineered: yes.

Source:

Monkeypox virus. Organism_taxid: 10244. Gene: f8l. Expressed in: insect expression vector pbluebachsgca1. Expression_system_taxid: 1944735. Gene: a22r. Gene: e4r. Synthetic: yes. DNA molecule.

Authors:

Y.Xu,Y.Wu,Y.Zhang,R.Fan,Y.Yang,D.Li,B.Yang,Z.Zhang,C.Dong

Key ref:

Y.X.Xu et al. Structure of DNA replication machinery from human mon virus. To be published, . PubMed id: 36973539

Date:

12-Dec-22 Release date: 31-Jan-24

Protein chain

A0A7H0DN44  (DPOL_MONPV) -  DNA polymerase from Monkeypox virus

Seq: 1006 a.a.

Struc: 1004 a.a.*

Protein chain

A0A7H0DNC0  (PG148_MONPV) -  DNA polymerase processivity factor component OPG148 from Monkeypox virus

Seq: 426 a.a.

Struc: 426 a.a.

Protein chain

M1LL92  (UNG_MONPV) -  Uracil-DNA glycosylase from Monkeypox virus

Seq: 218 a.a.

Struc: 218 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

A-T-G-G-T-A-G-G-G-G-A-A-G-G-A-T-C-G 18 bases

C-G-A-T-C-C-T-T-C-C-C-C-T-A-C 15 bases

Enzyme reactions

• Enzyme class 2: Chain A: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: Chain B: E.C.3.2.2.27 - uracil-DNA glycosylase.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site