PDBsum entry 8hkh

Toxin

PDB id: 8hkh

Contents

Protein chains

420 a.a.

134 a.a.

Metals

_ZN ×2

Waters ×26

PDB id:

8hkh

Name:

Toxin

Title:

Crystal structure of the lc/a1-darpin18 complex

Structure:

Bont/a. Chain: a, b. Engineered: yes. Designed ankyrin repeat protein 18. Chain: c, d. Synonym: darpin18. Engineered: yes

Source:

Clostridium botulinum. Organism_taxid: 1491. Gene: a. Expressed in: escherichia coli k-12. Expression_system_taxid: 83333. Synthetic construct. Organism_taxid: 32630. Expression_system_taxid: 83333

Resolution:

2.70Å R-factor: 0.264 R-free: 0.306

Authors:

Y.Wu,O.Leka,R.Kammerer

Key ref:

O.Leka et al. A darpin increases the catalytic activity of bont/a1. To be published, . PubMed id: 38110403

Date:

26-Nov-22 Release date: 29-Nov-23

Protein chains

P0DPI1  (BXA1_CLOBH) -  Botulinum neurotoxin type A from Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)

Seq: 1296 a.a.

Struc: 420 a.a.*

Protein chains

No UniProt id for this chain

Struc: 134 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.4.24.69 - bontoxilysin.
• Reaction: Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates.
• Cofactor: Zn(2+)