PDBsum entry 8hj8

Hydrolase

PDB id: 8hj8

Contents

Protein chain

606 a.a.

Ligands

NAG ×3

G2F

SHG

GOL ×5

ACT ×11

SO4 ×3

Waters ×629

PDB id:

8hj8

Name:

Hydrolase

Title:

Crystal structure of barley exohydrolase isoform exoi e220a mutant in complex with 2-deoxy-2-fluoro-d-glucopyranosides

Structure:

Glyco_hydro_3 domain-containing protein. Chain: a. Engineered: yes. Mutation: yes. Other_details: this protein sequence contains the expression tag (his-3 to ala-0), and engineered mutation residue is e220a. Whole protein sequence was confirmed by DNA sequencing.

Source:

Hordeum vulgare. Organism_taxid: 4513. Expressed in: komagataella pastoris. Expression_system_taxid: 4922

Resolution:

1.95Å R-factor: 0.146 R-free: 0.184

Authors:

S.Luang,V.A.Streltsov,M.Hrmova

Key ref:

S.Luang et al. Crystal structure of barley exohydrolase isoform exoi mutant in complex with 2-Deoxy-2-Fluoro-D-Glucopyrano. To be published, . PubMed id: 40348901

Date:

22-Nov-22 Release date: 25-Dec-24

Protein chain

Q9XEI3  (Q9XEI3_HORVV) -  Beta-D-glucan exohydrolase isoenzyme ExoI from Hordeum vulgare subsp. vulgare

Seq: 630 a.a.

Struc: 606 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)