PDBsum entry 8hj4

Hydrolase/RNA/antimicrobial protein

PDB id: 8hj4

Contents

Protein chains

1033 a.a.

130 a.a.

DNA/RNA

PDB id:

8hj4

Name:

Hydrolase/RNA/antimicrobial protein

Title:

Cryoem structure of an anti-crispr protein acriic5 bound to nme1cas9- sgrna complex

Structure:

Crispr-associated endonuclease cas9. Chain: a. Engineered: yes. Sgrna. Chain: b. Engineered: yes. Phage protein. Chain: c. Engineered: yes

Source:

Neisseria meningitidis serogroup c (strain 8013). Organism_taxid: 604162. Gene: cas9, nmv_1993. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Neisseria meningitidis 8013. Simonsiella muelleri atcc 29453.

Authors:

Y.Wang,W.Sun

Key ref:

Y.Wang and w.sun Cryoem structure of an anti-Crispr protein acriic5 bo nme1cas9-Sgrna complex. To be published, . PubMed id: 36744495

Date:

22-Nov-22 Release date: 25-Jan-23

Protein chain

C9X1G5  (CAS9_NEIM8) -  CRISPR-associated endonuclease Cas9 from Neisseria meningitidis serogroup C (strain 8013)

Seq: 1082 a.a.

Struc: 1033 a.a.

Protein chain

V9H5N5  (V9H5N5_9NEIS) -  Phage protein from Simonsiella muelleri ATCC 29453

Seq: 130 a.a.

Struc: 130 a.a.

DNA/RNA chain

U-A-A-C-U-U-U-A-C-G-U-U-G-U-A-G-C-U-C-C-C-U-U-U-C-U-C-G-A-A-A-G-A-G-A-A-C-C-G- 113 bases

Enzyme reactions

• Enzyme class: Chain A: E.C.3.1.-.-