PDBsum entry 8hj3

Oxidoreductase

PDB id: 8hj3

Contents

Protein chain

416 a.a.

Ligands

NAP

PDB id:

8hj3

Name:

Oxidoreductase

Title:

Cryoem structure of glutamate dehydrogenase from thermococcus profundus in complex with NADP

Structure:

Glutamate dehydrogenase. Chain: a. Engineered: yes

Source:

Thermococcus profundus. Organism_taxid: 49899. Gene: gdha. Expressed in: escherichia coli. Expression_system_taxid: 562

Authors:

T.Wakabayashi,M.Oide,T.Kato,M.Nakasako

Key ref:

T.Wakabayashi et al. Coenzyme-Binding pathway on glutamate dehydrogenase suggested from multiple binding sites visualized by cryo-Electron microscopy. To be published, . PubMed id: 37682540

Date:

22-Nov-22 Release date: 08-Feb-23

Protein chain

O74024  (DHE3_THEPR) -  Glutamate dehydrogenase from Thermococcus profundus

Seq: 419 a.a.

Struc: 416 a.a.

Enzyme reactions

• Enzyme class: E.C.1.4.1.3 - glutamate dehydrogenase [NAD(P)(+)].
• Reaction:
  1. L-glutamate + NAD⁺ + H2O = 2-oxoglutarate + NH4⁺ + NADH + H⁺
  2. L-glutamate + NADP⁺ + H2O = 2-oxoglutarate + NH4⁺ + NADPH + H⁺

L-glutamate + NAD(+) + H2O (Bound ligand (Het Group name = NAP) matches with 91.67% similarity) = 2-oxoglutarate + NH4(+) + NADH + H(+)

L-glutamate + NADP(+) + H2O (Bound ligand (Het Group name = NAP) corresponds exactly) = 2-oxoglutarate + NH4(+) + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site