PDBsum entry 8hfd

Hydrolase

PDB id: 8hfd

Contents

Protein chains

452 a.a.

Ligands

PEG ×6

Metals

_ZN ×8

Waters ×926

PDB id:

8hfd

Name:

Hydrolase

Title:

Crystal structure of allantoinase from e. Coli bl21

Structure:

Allantoinase. Chain: a, b, c, d. Synonym: allantoin-utilizing enzyme. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: allb, bmt91_19075, e2127_17875, e2128_18450, e2132_17365, e4t14_14655, e6d34_22765, elt21_17110. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.07Å R-factor: 0.206 R-free: 0.249

Authors:

E.S.Lin,H.Y.Huang,P.C.Yang,H.W.Liu,C.Y.Huang

Key ref:

Y.H.Huang et al. (2023). Crystal structure of allantoinase from escherichia co bl21: a molecular insight into a role of the active s loops in catalysis.. Molecules, 28, . PubMed id: 36677881

Date:

10-Nov-22 Release date: 18-Oct-23

Protein chains

P77671  (ALLB_ECOLI) -  Allantoinase from Escherichia coli (strain K12)

Seq: 453 a.a.

Struc: 452 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.2.5 - allantoinase.
• Pathway: AMP Catabolism
• Reaction: (S)-allantoin + H2O = allantoate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site