PDBsum entry 8hf1

RNA binding protein/RNA

PDB id: 8hf1

Contents

Protein chains

1561 a.a.

16 a.a.

126 a.a.

DNA/RNA

PDB id:

8hf1

Name:

RNA binding protein/RNA

Title:

Dmdcr-2/r2d2/loqspd with 19bp-dsrna in trimer state

Structure:

Dicer-2, isoform a. Chain: a, d, g. Synonym: fi15132p1. Ec: 3.1.21.1,3.1.26.-,3.1.26.3,3.6.1.3. Engineered: yes. Loquacious, isoform d. Chain: b. Engineered: yes. Ld06392p.

Source:

Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: dcr-2, cg6493, dcr, dcr, dcr-2, dcr-2, dcr-2-ra, dcr2, dcr2, dcr2, ddcr2, dic2, dicer, dicer, dicer, dicer-2, dicer-2, dicer2, dicer2, dmdcr-2, dmel\cg6493, cg6493, dmel_cg6493. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: loqs, cg6866, dmel\cg6866, drax, loq, loqs, loqs, loqs-pd,

Authors:

S.Su,J.Wang,J.Ma

Key ref:

T.Deng et al. (2023). Structural mechanism of r2d2 and loqs-Pd synergistic modulation on dmdcr-2 oligomers.. Nat commun, 14, 5228. PubMed id: 37633971

Date:

09-Nov-22 Release date: 06-Sep-23

Protein chains

A1ZAW0  (A1ZAW0_DROME) -  Endoribonuclease Dcr-2 from Drosophila melanogaster

Seq: 1722 a.a.

Struc: 1561 a.a.*

Protein chain

Q9VJY9  (Q9VJY9_DROME) -  Protein Loquacious from Drosophila melanogaster

Seq: 465 a.a.

Struc: 16 a.a.*

Protein chains

Q9VLW8  (Q9VLW8_DROME) -  LD06392p from Drosophila melanogaster

Seq: 311 a.a.

Struc: 126 a.a.

* PDB and UniProt seqs differ at 16 residue positions (black crosses)

DNA/RNA chains

G-A-G-A-C-U-U-G-G 9 bases

C-C-A-A-G-U-C-U-C-U-U 11 bases

G-A-G-A-C-U-U-G-G 9 bases

C-C-A-A-G-U-C-U-C-U-U 11 bases

G-A-G-A-C-U-U-G-G 9 bases

C-C-A-A-G-U-C-U-C-U-U 11 bases

Enzyme reactions

• Enzyme class: Chains A, D, G: E.C.3.1.26.3 - ribonuclease Iii.
• Reaction: Endonucleolytic cleavage to 5'-phosphomonoester.