PDBsum entry 8hdi

Oxidoreductase

PDB id: 8hdi

Contents

Protein chains

236 a.a.

249 a.a.

Waters ×736

PDB id:

8hdi

Name:

Oxidoreductase

Title:

Brucella melitensis 7-alpha-hydroxysteroid dehydrogenase mutant: 1-53 truncation/k262t

Structure:

7-alpha-hydroxysteroid dehydrogenase. Chain: a, b, c, d. Fragment: 1-53 truncation. Engineered: yes. Mutation: yes. Other_details: sequence reference for wp_014489477 is not available in uniprot at the time of biocuration. Current sequence reference is from uniprot id q8yin7.

Source:

Brucella melitensis biotype 1 (strain 16m / atcc 23456 / nctc 10094). Organism_taxid: 224914. Gene: bmei0406. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.99Å R-factor: 0.175 R-free: 0.212

Authors:

Z.Y.Liu,R.Z.Zhang

Key ref:

Z.Y.Liu and r.z.zhang Structure of 7-Alpha-Hydroxysteroid dehydrogenase. To be published, .

Date:

04-Nov-22 Release date: 03-Jan-24

Protein chains

Q8YIN7  (Q8YIN7_BRUME) -  7-alpha-hydroxysteroid dehydrogenase from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

Seq: 304 a.a.

Struc: 236 a.a.*

Protein chain

Q8YIN7  (Q8YIN7_BRUME) -  7-alpha-hydroxysteroid dehydrogenase from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

Seq: 304 a.a.

Struc: 249 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C: E.C.1.1.1.159 - 7alpha-hydroxysteroid dehydrogenase.
• Reaction: cholate + NAD⁺ = 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site