PDBsum entry 8hdf

Biosynthetic protein

PDB id

8hdf

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Contents

			Protein chain

					539 a.a.

			Ligands

					PLM


					ANP

			Waters ×70

PDB id:

8hdf

Links

Name:

Biosynthetic protein

Title:

Full length crystal structure of mycobacterium tuberculosis fadd23 in complex with anp and plm

Structure:

Long-chain-fatty-acid--amp ligase fadd23. Chain: a. Synonym: faal23,long-chain fatty acid adenylyltransferase fadd23, long-chain-fatty-acid adenylase/transferase fadd23. Engineered: yes

Source:

Mycobacterium tuberculosis (strain atcc 25618 / h37rv). Organism_taxid: 83332. Gene: fadd23, rv3826. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.24Å

R-factor:

0.208

R-free:

0.256

Authors:

M.R.Yan,X.Liu,W.Zhang,Z.H.Rao

Key ref:

W.Zhang and m.r.yan The key roles of mycobacterium tuberculosis fadd23 c-Terminal domain in catalytic mechanism. To be published, . PubMed id: 36896429

Date:

04-Nov-22

Release date:

15-Feb-23

PROCHECK

	Headers

	References

Protein chain

P9WQ47 (FAC23_MYCTU) - Long-chain-fatty-acid--AMP ligase FadD23 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: Struc:

Seq: Struc:					584 a.a. 539 a.a.

Key:

PfamA domain

Secondary structure



		Enzyme reactions

Enzyme class:

E.C.6.2.1.57 - long-chain fatty acid adenylase/transferase FadD23.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	holo-[(hydroxy)phthioceranic acid synthase] + octadecanoate + ATP = octadecanoyl-[(hydroxy)phthioceranic acid synthase] + AMP + diphosphate
2.	holo-[(hydroxy)phthioceranic acid synthase] + hexadecanoate + ATP = hexadecanoyl-[(hydroxy)phthioceranic acid synthase] + AMP + diphosphate

holo-[(hydroxy)phthioceranic acid synthase]	+	octadecanoate	+	ATP	=	octadecanoyl-[(hydroxy)phthioceranic acid synthase] Bound ligand (Het Group name = ANP) matches with 74.19% similarity	+	AMP	+	diphosphate

holo-[(hydroxy)phthioceranic acid synthase]

hexadecanoate
Bound ligand (Het Group name = PLM)
corresponds exactly

ATP

hexadecanoyl-[(hydroxy)phthioceranic acid synthase]
Bound ligand (Het Group name = ANP)
matches with 74.19% similarity

AMP

diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site