PDBsum entry 8hdd

Oxidoreductase

PDB id: 8hdd

Contents

Protein chains

530 a.a.

121 a.a.

117 a.a.

Ligands

FAD

F3S

HEM

PDB id:

8hdd

Name:

Oxidoreductase

Title:

Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase

Structure:

Glucose dehydrogenase. Chain: a. Engineered: yes. Other_details: gb:aan39686.1. Glucose dehydrogenase beta subunit. Chain: b. Engineered: yes. Other_details: gb:aaq06608.1. Twin-arginine translocation pathway signal.

Source:

Burkholderia cepacia. Organism_taxid: 292. Gene: gdhalpha. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: bmulj_04411.

Resolution:

3.00Å R-factor: 0.278 R-free: 0.323

Authors:

H.Yoshida,K.Sode

Key ref:

J.Okuda-Shimazaki et al. (2022). Microgravity environment grown crystal structure info based engineering of direct electron transfer type gl dehydrogenase. Commun biol, 5, 1334. PubMed id: 36473944

Date:

04-Nov-22 Release date: 14-Dec-22

Protein chain

Q8GQE7  (Q8GQE7_BURCE) -  Glucose dehydrogenase from Burkholderia cepacia

Seq: 539 a.a.

Struc: 530 a.a.

Protein chain

Q71JE9  (Q71JE9_BURCE) -  Glucose dehydrogenase beta subunit from Burkholderia cepacia

Seq: 425 a.a.

Struc: 121 a.a.

Protein chain

A0A0H3KLY3  (A0A0H3KLY3_BURM1) -  Twin-arginine translocation pathway signal from Burkholderia multivorans (strain ATCC 17616 / 249)

Seq: 168 a.a.

Struc: 117 a.a.

Enzyme reactions

• Enzyme class: Chain A: E.C.1.1.5.9 - glucose 1-dehydrogenase (FAD, quinone).
• Reaction: a quinone + D-glucose = D-glucono-1,5-lactone + a quinol
• Cofactor: FAD

FAD (Bound ligand (Het Group name = FAD) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site