PDBsum entry 8h5k

Hydrolase

PDB id: 8h5k

Contents

Protein chain

260 a.a.

Waters ×329

PDB id:

8h5k

Name:

Hydrolase

Title:

Crystal structure of petase n37d/s121e/r132e/a171c/a180v/p181v/d186h/s193c/r224e/n233c/s242t/n246 d/s282c mutant from ideonella sakaiensis

Structure:

Poly(ethylene terephthalate) hydrolase. Chain: a. Synonym: pet hydrolase,petase,pet-digesting enzyme. Engineered: yes

Source:

Ideonella sakaiensis. Organism_taxid: 1547922. Gene: isf6_4831. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.20Å R-factor: 0.160 R-free: 0.187

Authors:

S.H.Lee,H.Seo,K.-J.Kim

Key ref:

S.H.Lee et al. A case of balance engineering exhibits kinetic relati between mesophilic and thermophilic poly(ethylene terephthalate) depolymerases.. To be published, .

Date:

13-Oct-22 Release date: 25-Oct-23

Protein chain

A0A0K8P6T7  (PETH_IDESA) -  Poly(ethylene terephthalate) hydrolase from Piscinibacter sakaiensis

Seq: 290 a.a.

Struc: 260 a.a.*

* PDB and UniProt seqs differ at 16 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.1.101 - poly(ethylene terephthalate) hydrolase.
• Reaction: (ethylene terephthalate)(n) + H2O = (ethylene terephthalate)(n-1) + 4-[(2-hydroxyethoxy)carbonyl]benzoate + H⁺