PDBsum entry 8h4f

Hydrolase

PDB id: 8h4f

Contents

Protein chains

319 a.a.

Ligands

FMT ×7

DSG ×2

Metals

_MG ×4

Waters ×451

PDB id:

8h4f

Name:

Hydrolase

Title:

Blasnase-t13a/p55f with d-asn

Structure:

L-asparaginase. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Bacillus paralicheniformis. Organism_taxid: 1648923. Gene: b4121_3474. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.90Å R-factor: 0.191 R-free: 0.211

Authors:

F.Lu,W.Wang,H.Chi,T.Ran

Key ref:

F.Lu et al. Structure-Based rational design of bacillus lichenifo l-Asparaginase with low/no d-Asparaginase activity fo safer enzyme. To be published, .

Date:

10-Oct-22 Release date: 08-Nov-23

Protein chains

A0A6I7U6Y2  () -  L-asparaginase from Bacillus paralicheniformis

Seq: 322 a.a.

Struc: 319 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)