PDBsum entry 8h40

Transcription

PDB id: 8h40

Contents

Protein chains

1077 a.a.

1217 a.a.

226 a.a.

620 a.a.

58 a.a.

314 a.a.

196 a.a.

DNA/RNA

PDB id:

8h40

Name:

Transcription

Title:

Cryo-em structure of the transcription activation complex ntca-tac

Structure:

DNA (125-mer). Chain: 1. Engineered: yes. DNA (125-mer). Chain: 2. Engineered: yes. DNA-directed RNA polymerase subunit beta. Chain: a. Synonym: rnap subunit beta,RNA polymerase subunit beta,transcriptase

Source:

Synthetic: yes. Anabaena. Organism_taxid: 1163. Strain: pcc 7120 / sag 25.82 / utex 2576. Gene: rpob, alr1594. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: rpoc2, alr1596. Gene: rpoa, all4191.

Authors:

S.J.Han,Y.L.Jiang,L.L.You,L.Q.Shen,X.X.Wu,F.Yang,W.W.Kong,Z.P.Chen, Y.Zhang,C.Z.Zhou

Key ref:

S.J.Han et al. Cryo-Em structure of the transcription activation com ntca-Tac. To be published, . PubMed id: 38177666

Date:

09-Oct-22 Release date: 04-Oct-23

Protein chain

P22703  (RPOB_NOSS1) -  DNA-directed RNA polymerase subunit beta from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

Seq: 1131 a.a.

Struc: 1077 a.a.

Protein chain

P22705  (RPOC2_NOSS1) -  DNA-directed RNA polymerase subunit beta' from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

Seq: 1355 a.a.

Struc: 1217 a.a.

Protein chains

Q8YPK3  (RPOA_NOSS1) -  DNA-directed RNA polymerase subunit alpha from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

Seq: 315 a.a.

Struc: 226 a.a.

Protein chain

P22704  (RPOC1_NOSS1) -  DNA-directed RNA polymerase subunit gamma from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

Seq: 625 a.a.

Struc: 620 a.a.

Protein chain

Q8YNB9  (RPOZ_NOSS1) -  DNA-directed RNA polymerase subunit omega from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

Seq: 78 a.a.

Struc: 58 a.a.

Protein chain

P26683  (SIGA_NOSS1) -  RNA polymerase sigma factor SigA from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

Seq: 390 a.a.

Struc: 314 a.a.

Protein chains

P0A4U6  (NTCA_NOSS1) -  Global nitrogen regulator from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

Seq: 223 a.a.

Struc: 196 a.a.

DNA/RNA chains

A-T-T-T-T-G-T-A-G-C-T-A-C-T-T-A-T-A-C-A-A-A-A-T-T-C-A-G-G-A-A-A-A-T-T-T-T-T-C- 66 bases

T-G-C-A-T-C-C-G-T-G-A-A-A-C-A-G-A-A-A-A-A-T-T-T-T-C-C-T-G-A-A-T-T-T-T-G-T-A-T- 54 bases

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site