PDBsum entry 8h0v

Transcription

PDB id: 8h0v

Contents

Protein chains

1412 a.a.

1161 a.a.

263 a.a.

168 a.a.

213 a.a.

84 a.a.

171 a.a.

133 a.a.

111 a.a.

66 a.a.

113 a.a.

45 a.a.

97 a.a.

83 a.a.

105 a.a.

95 a.a.

78 a.a.

73 a.a.

DNA/RNA

Metals

_MG

_ZN ×8

PDB id:

8h0v

Name:

Transcription

Title:

RNA polymerase ii transcribing a chromatosome (type i)

Structure:

DNA-directed RNA polymerase subunit. Chain: a. DNA-directed RNA polymerase subunit beta. Chain: b. RNA polymerase ii third largest subunit b44, part of central core. Chain: c. RNA polymerase ii subunit b32. Chain: d.

Source:

Komagataella phaffii. Organism_taxid: 460519. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Homo sapiens. Human. Organism_taxid: 9606. Gene: h3c1.

Authors:

R.Hirano,H.Ehara,K.Tomoya,Y.Takizawa,S.Sekine,H.Kurumizaka

Key ref:

R.Hirano et al. (2022). Structural basis of RNA polymerase ii transcription o chromatosome containing linker histone h1.. Nat commun, 13, 7287. PubMed id: 36435862

Date:

30-Sep-22 Release date: 07-Dec-22

Protein chain

C4R4Y0  (C4R4Y0_KOMPG) -  DNA-directed RNA polymerase subunit from Komagataella phaffii (strain GS115 / ATCC 20864)

Seq: 1743 a.a.

Struc: 1412 a.a.

Protein chain

C4QZQ7  (C4QZQ7_KOMPG) -  DNA-directed RNA polymerase subunit beta from Komagataella phaffii (strain GS115 / ATCC 20864)

Seq: 1227 a.a.

Struc: 1161 a.a.

Protein chain

C4R7L2  (C4R7L2_KOMPG) -  RNA polymerase II third largest subunit B44, part of central core from Komagataella phaffii (strain GS115 / ATCC 20864)

Seq: 304 a.a.

Struc: 263 a.a.

Protein chain

C4R2U9  (C4R2U9_KOMPG) -  RNA polymerase II subunit B32 from Komagataella phaffii (strain GS115 / ATCC 20864)

Seq: 186 a.a.

Struc: 168 a.a.

Protein chain

C4R3P8  (C4R3P8_KOMPG) -  DNA-directed RNA polymerases I, II, and III subunit RPABC1 from Komagataella phaffii (strain GS115 / ATCC 20864)

Seq: 214 a.a.

Struc: 213 a.a.

Protein chain

C4R1V1  (C4R1V1_KOMPG) -  RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III from Komagataella phaffii (strain GS115 / ATCC 20864)

Seq: 155 a.a.

Struc: 84 a.a.

Protein chain

C4R9A1  (C4R9A1_KOMPG) -  DNA-directed RNA polymerase subunit from Komagataella phaffii (strain GS115 / ATCC 20864)

Seq: 171 a.a.

Struc: 171 a.a.

Protein chain

C4R273  (C4R273_KOMPG) -  DNA-directed RNA polymerases I, II, and III subunit RPABC3 from Komagataella phaffii (strain GS115 / ATCC 20864)

Seq: 145 a.a.

Struc: 133 a.a.

Protein chain

F2QPE6  (F2QPE6_KOMPC) -  DNA-directed RNA polymerase subunit from Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1)

Seq: 115 a.a.

Struc: 111 a.a.

Protein chain

C4R009  (C4R009_KOMPG) -  DNA-directed RNA polymerases I, II, and III subunit RPABC5 from Komagataella phaffii (strain GS115 / ATCC 20864)

Seq: 72 a.a.

Struc: 66 a.a.

Protein chain

C4R3Z5  (C4R3Z5_KOMPG) -  RNA polymerase II subunit B12.5 from Komagataella phaffii (strain GS115 / ATCC 20864)

Seq: 118 a.a.

Struc: 113 a.a.

Protein chain

F2QMI1  (F2QMI1_KOMPC) -  RNA polymerase subunit ABC10-alpha from Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1)

Seq: 72 a.a.

Struc: 45 a.a.

Protein chains

P68431  (H31_HUMAN) -  Histone H3.1 from Homo sapiens

Seq: 136 a.a.

Struc: 97 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 83 a.a.

Protein chains

P04908  (H2A1B_HUMAN) -  Histone H2A type 1-B/E from Homo sapiens

Seq: 130 a.a.

Struc: 105 a.a.

Protein chains

P06899  (H2B1J_HUMAN) -  Histone H2B type 1-J from Homo sapiens

Seq: 126 a.a.

Struc: 95 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 78 a.a.

Protein chain

P16403  (H12_HUMAN) -  Histone H1.2 from Homo sapiens

Seq: 213 a.a.

Struc: 73 a.a.

DNA/RNA chains

A-G-C-A-A-U-A-G-G-A-G-C-U-U 14 bases

T-A-T-G-A-A-T-T-T-C-G-C-G-A-C-A-C-A-A-G-G-C-C-T-G-G-A-T-G-T-A-T-A-T-A-T-C-T-G- 210 bases

C-T-T-T-A-A-A-G-C-A-A-A-C-G-G-T-C-C-A-C-T-T-G-T-G-T-T-T-G-G-T-G-T-G-T-T-T-G-G- 201 bases

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site