PDBsum entry 8h0t

Transferase

PDB id: 8h0t

Contents

Protein chain

184 a.a.

Ligands

SAH

Waters ×191

PDB id:

8h0t

Name:

Transferase

Title:

Crystal structure of mnmm from b. Subtilis complexed with sah (1.17 a)

Structure:

Putative rrna methylase ytqb. Chain: a. Engineered: yes

Source:

Bacillus subtilis subsp. Subtilis str. 168. Organism_taxid: 224308. Strain: 168. Gene: ytqb, bsu30490. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.17Å R-factor: 0.136 R-free: 0.167

Authors:

J.Kim,J.Lee,G.Cho

Key ref:

J.Kim et al. Crystal structure of mnmm from b. Subtilis complexed sah (1.17 a). To be published, . PubMed id: 36762482

Date:

30-Sep-22 Release date: 25-Jan-23

Protein chain

O34614  (YTQB_BACSU) -  tRNA (mnm(5)s(2)U34)-methyltransferase from Bacillus subtilis (strain 168)

Seq: 194 a.a.

Struc: 184 a.a.

Enzyme reactions

• Enzyme class: E.C.2.1.1.61 - tRNA 5-(aminomethyl)-2-thiouridylate-methyltransferase.
• Reaction: 5-aminomethyl-2-thiouridine₃₄ in tRNA + S-adenosyl-L-methionine = 5-methylaminomethyl-2-thiouridine₃₄ in tRNA + S-adenosyl-L-homocysteine + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site