PDBsum entry 8gtk

Immune system

PDB id: 8gtk

Contents

Protein chains

324 a.a.

236 a.a.

PDB id:

8gtk

Name:

Immune system

Title:

Crystal structure of ipah7.8-lrr and gsdmb isoform-1 complex

Structure:

Gsdmb isoform-1. Chain: a. Engineered: yes. Probable e3 ubiquitin-protein ligase ipah7.8. Chain: b. Synonym: probable ring-type e3 ubiquitin transferase ipah7.8. Engineered: yes

Source:

Homo sapiens. Organism_taxid: 9606. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Shigella flexneri. Organism_taxid: 623. Gene: ipah7.8, cp0078, pwr501_0084, sflp133. Expression_system_taxid: 469008

Resolution:

3.10Å R-factor: 0.268 R-free: 0.290

Authors:

X.Zhong,Y.J.Hou,J.Ding

Key ref:

X.Zhong et al. Structural mechanisms for regulation of gsdmb pore-Fo activity.. Nature, . PubMed id: 36991125

Date:

08-Sep-22 Release date: 12-Apr-23

Protein chain

Q8TAX9  (GSDMB_HUMAN) -  Gasdermin-B from Homo sapiens

Seq: 416 a.a.

Struc: 324 a.a.

Protein chain

P18014  (IPA7_SHIFL) -  E3 ubiquitin-protein ligase ipaH7.8 from Shigella flexneri

Seq: 565 a.a.

Struc: 236 a.a.

Enzyme reactions

• Enzyme class: Chain B: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine