PDBsum entry 8grh

Oxidoreductase

PDB id: 8grh

Contents

Protein chains

324 a.a.

335 a.a.

Ligands

CIT

Waters ×33

PDB id:

8grh

Name:

Oxidoreductase

Title:

Crystal structure of a constitutively active mutant of the alpha gamma heterodimer of human idh3 in complex with cit

Structure:

Human idh3 alpha subunit. Chain: a. Engineered: yes. Mutation: yes. Isocitrate dehydrogenase [nad] subunit gamma, mitochondrial. Chain: b. Synonym: NAD-idh gamma subunit, isocitric dehydrogenase subunit gamma,NAD(+)-specific icdh subunit gamma.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: idh3g. Expression_system_taxid: 562

Resolution:

2.51Å R-factor: 0.219 R-free: 0.259

Authors:

P.Sun,X.Chen,J.Ding

Key ref:

X.Chen et al. (2022). Structures of a constitutively active mutant of human reveal new insights into the mechanisms of allosteric activation and the catalytic reaction.. J.Biol.Chem., 298, 02695. PubMed id: 36375638 DOI: 10.1016/J.JBC.2022.102695

Date:

01-Sep-22 Release date: 30-Nov-22

Protein chain

P50213  (IDH3A_HUMAN) -  Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial from Homo sapiens

Seq: 366 a.a.

Struc: 324 a.a.*

Protein chain

P51553  (IDH3G_HUMAN) -  Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial from Homo sapiens

Seq: 393 a.a.

Struc: 335 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.1.1.1.41 - isocitrate dehydrogenase (NAD(+)).
• Pathway: Citric acid cycle
• Reaction: D-threo-isocitrate + NAD⁺ = 2-oxoglutarate + CO2 + NADH

D-threo-isocitrate + NAD(+) = 2-oxoglutarate (Bound ligand (Het Group name = CIT) matches with 64.29% similarity) + CO2 + NADH

• Cofactor: Mn(2+) or Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site