PDBsum entry 8gcc

Isomerase

PDB id: 8gcc

Contents

Protein chains

707 a.a.

DNA/RNA

Ligands

YWX ×2

PDB id:

8gcc

Name:

Isomerase

Title:

T. Cruzi topoisomerase ii alpha bound to dsdna and the covalent inhibitor ct1

Structure:

DNA topoisomerase 2. Chain: a, b. Engineered: yes. DNA (28-mer). Chain: c, d. Engineered: yes

Source:

Trypanosoma cruzi strain cl brener. Organism_taxid: 353153. Gene: tc00.1047053508699.10. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Synthetic: yes. Escherichia coli. Organism_taxid: 562

Authors:

A.Schenk,C.Deniston,J.Noeske

Key ref:

S.P.S.Rao et al. (2023). Cyanotriazoles are selective topoisomerase ii poisons rapidly cure trypanosome infections.. Science, 380, 1349. PubMed id: 37384702

Date:

01-Mar-23 Release date: 12-Jul-23

Protein chains

Q4DE53  (Q4DE53_TRYCC) -  DNA topoisomerase 2 from Trypanosoma cruzi (strain CL Brener)

Seq: 1476 a.a.

Struc: 707 a.a.

DNA/RNA chains

G-A-T-A-A-C-A-A-T-G-A-G-C-T-C-A-T-T-G-T-T-A-T-C 24 bases

G-A-T-A-A-C-A-A-T-G-A-G-C-T-C-A-T-T-G-T-T-A-T-C 24 bases

Enzyme reactions

• Enzyme class: E.C.5.6.2.2 - Dna topoisomerase (ATP-hydrolyzing).