PDBsum entry 8fbz

Biosynthetic protein

PDB id: 8fbz

Contents

Protein chains

460 a.a.

Ligands

SO4 ×6

GOL

Waters ×1046

PDB id:

8fbz

Name:

Biosynthetic protein

Title:

Crystal structure of apo human glutathione synthetase y270e

Structure:

Glutathione synthetase. Chain: b, a. Synonym: gsh synthetase,gsh-s,glutathione synthase. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gss. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.59Å R-factor: 0.160 R-free: 0.189

Authors:

S.M.Stanford,E.Santelli,B.Sankaran,R.Murali,N.Bottini

Key ref:

S.M.Stanford et al. (2024). Targeting prostate tumor low-Molecular weight tyrosin phosphatase for oxidation-Sensitizing therapy.. Sci adv, 10, g7887. PubMed id: 38295166

Date:

30-Nov-22 Release date: 08-May-24

Protein chains

P48637  (GSHB_HUMAN) -  Glutathione synthetase from Homo sapiens

Seq: 474 a.a.

Struc: 460 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.6.3.2.3 - glutathione synthase.
• Reaction: gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site