PDBsum entry 8edh

Transferase

PDB id: 8edh

Contents

Protein chains

255 a.a.

Ligands

WGK

Waters ×158

PDB id:

8edh

Name:

Transferase

Title:

Identification of a class of wnk isoform-specific inhibitors through high-throughput screening

Structure:

Serine/threonine-protein kinase wnk3. Chain: a, c. Synonym: protein kinase lysine-deficient 3,protein kinase with no lysine 3. Engineered: yes. Mutation: yes. Other_details: kinase domain of wnk3 with s308a mutation

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: wnk3, kiaa1566, prkwnk3. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.

Resolution:

3.11Å R-factor: 0.186 R-free: 0.291

Authors:

R.Akella,E.J.Goldsmith

Key ref:

J.Chlebowicz et al. (2023). Identification of a class of wnk isoform-Specific inh through high-Throughput screening.. Drug des devel ther, 17, 93. PubMed id: 36712947

Date:

04-Sep-22 Release date: 01-Mar-23

Protein chains

Q9BYP7  (WNK3_HUMAN) -  Serine/threonine-protein kinase WNK3 from Homo sapiens

Seq: 1800 a.a.

Struc: 255 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site