PDBsum entry 8dfc

Metal binding protein, oxidoreductase

PDB id: 8dfc

Contents

Protein chains

478 a.a.

522 a.a.

274 a.a.

Ligands

ICS ×2

HCA ×2

CLF ×2

SF4

ADP ×2

ALF ×2

Metals

_FE ×2

_MG ×2

Waters ×483

PDB id:

8dfc

Name:

Metal binding protein, oxidoreductase

Title:

Cryoem structure of the 1:1 adp-tetrafluoroaluminate stabilized nitrogenase complex from azotobacter vinelandii

Structure:

Nitrogenase molybdenum-iron protein alpha chain. Chain: a, c. Synonym: dinitrogenase,nitrogenase component i. Nitrogenase molybdenum-iron protein beta chain. Chain: b, d. Synonym: dinitrogenase, nitrogenase component i. Nitrogenase iron protein 1. Chain: e, f. Synonym: nitrogenase fe protein 1,nitrogenase component ii,

Source:

Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354

Authors:

R.A.Warmack,D.C.Rees

Key ref:

R.A.Warmack and d.c.rees Anaerobic single particle cryoem of nitrogenase. To be published, . PubMed id: 38575747

Date:

21-Jun-22 Release date: 28-Jun-23

Protein chains

P07328  (NIFD_AZOVI) -  Nitrogenase molybdenum-iron protein alpha chain from Azotobacter vinelandii

Seq: 492 a.a.

Struc: 478 a.a.

Protein chains

P07329  (NIFK_AZOVI) -  Nitrogenase molybdenum-iron protein beta chain from Azotobacter vinelandii

Seq: 523 a.a.

Struc: 522 a.a.

Protein chains

P00459  (NIFH1_AZOVI) -  Nitrogenase iron protein 1 from Azotobacter vinelandii

Seq: 290 a.a.

Struc: 274 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.1.18.6.1 - nitrogenase.
• Pathway: Nitrogenase
• Reaction: N2 + 8 reduced [2Fe-2S]-[ferredoxin] + 16 ATP + 16 H2O = H2 + 8 oxidized [2Fe-2S]-[ferredoxin] + 2 NH4⁺ + 16 ADP + 16 phosphate + 6 H⁺

N2 + 8 × reduced [2Fe-2S]-[ferredoxin] + 16 × ATP + 16 × H2O = H2 + 8 × oxidized [2Fe-2S]-[ferredoxin] + 2 × NH4(+) + 16 × ADP (Bound ligand (Het Group name = ADP) corresponds exactly) + 16 × phosphate + 6 × H(+)

• Cofactor: Iron-sulfur; Vanadium cation or Mo cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site