PDBsum entry 8da4

Protein binding

PDB id: 8da4

Contents

Protein chains

56 a.a.

56 a.a.

57 a.a.

53 a.a.

54 a.a.

Ligands

SO4

Waters ×108

PDB id:

8da4

Name:

Protein binding

Title:

Coevolved affibody-z domain pair ll1.C2

Structure:

Immunoglobulin g-binding protein a. Chain: a, c, e. Synonym: igg-binding protein a,staphylococcal protein a,spa. Engineered: yes. Mutation: yes. Affibody ll1.Fivm. Chain: b. Engineered: yes. Affibody ll1.Fivm.

Source:

Staphylococcus aureus. Organism_taxid: 1280. Gene: spa. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic construct. Organism_taxid: 32630. Expression_system_taxid: 562

Resolution:

1.92Å R-factor: 0.206 R-free: 0.247

Authors:

K.M.Jude,A.Yang,K.C.Garcia

Key ref:

A.Yang et al. Deploying synthetic coevolution and machine learning engineer protein-Protein interactions. Science, . PubMed id: 37499032

Date:

13-Jun-22 Release date: 26-Jul-23

Protein chains

P02976  (SPA_STAA8) -  Immunoglobulin G-binding protein A from Staphylococcus aureus (strain NCTC 8325 / PS 47)

Seq: 516 a.a.

Struc: 56 a.a.*

Protein chain

No UniProt id for this chain

Struc: 56 a.a.

Protein chain

P02976  (SPA_STAA8) -  Immunoglobulin G-binding protein A from Staphylococcus aureus (strain NCTC 8325 / PS 47)

Seq: 516 a.a.

Struc: 57 a.a.*

Protein chain

No UniProt id for this chain

Struc: 53 a.a.

Protein chain

No UniProt id for this chain

Struc: 54 a.a.

* PDB and UniProt seqs differ at 11 residue positions (black crosses)