PDBsum entry 8d0s

Transferase

PDB id: 8d0s

Contents

Protein chain

297 a.a.

Ligands

NAG-NAG-BMA-MAN

GAL-NAG-GAL

GDP

SO4 ×4

EDO ×3

Waters ×321

PDB id:

8d0s

Name:

Transferase

Title:

Human fut9 bound to gdp and lnnt

Structure:

4-galactosyl-n-acetylglucosaminide 3-alpha-l- fucosyltransferase 9. Chain: a. Synonym: fucosyltransferase 9,fucosyltransferase ix,fuc-tix,fuct-ix, galactoside 3-l-fucosyltransferase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: fut9. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293s

Resolution:

1.37Å R-factor: 0.159 R-free: 0.182

Authors:

R.Kadirvelraj,Z.A.Wood

Key ref:

R.Kadirvelraj et al. Structural basis for lewis antigen synthesis by the a 1,3-Fucosyltransferase fut9.. Nat chem biol, . PubMed id: 37202521

Date:

26-May-22 Release date: 24-May-23

Protein chain

Q9Y231  (FUT9_HUMAN) -  4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase 9 from Homo sapiens

Seq: 359 a.a.

Struc: 297 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.4.1.152 - 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase.
• Reaction: a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + GDP- beta-L-fucose = a beta-D-galactosyl-(1->4)-[alpha-L-fucosyl-(1->3)]-N- acetyl-beta-D-glucosaminyl derivative + GDP + H⁺

beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + GDP- beta-L-fucose = beta-D-galactosyl-(1->4)-[alpha-L-fucosyl-(1->3)]-N- acetyl-beta-D-glucosaminyl derivative + GDP (Bound ligand (Het Group name = GDP) corresponds exactly) + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site