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PDBsum entry 8cxy
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DNA binding protein/DNA
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PDB id
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8cxy
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PDB id:
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| Name: |
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DNA binding protein/DNA
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Title:
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Cama adenine methyltransferase complexed to cognate substrate DNA and inhibitor n6-(2-phenethyl)adenosine (compound 8)
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Structure:
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Site-specific DNA-methyltransferase (adenine-specific). Chain: a, b, c. Engineered: yes. DNA strand 1. Chain: d, f, h. Engineered: yes. DNA strand 2. Chain: e, g, i. Engineered: yes
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Source:
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Clostridioides difficile. Organism_taxid: 1496. Gene: bn1095_20232, bn1096_690041, bn1097_700039, e5f26_07025, e5f27_14470, e5f28_16275, e5f29_09620, e5f30_09400, e5f31_14185, e5f32_03810, e5f33_08870, e5f36_00990, e5f37_03060, e5f38_04245, e5f39_00835, e5f40_02785, e5f41_17405, e5f42_11180, e5f43_12285, e5f44_14865, e5f45_19095. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
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Resolution:
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2.19Å
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R-factor:
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0.175
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R-free:
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0.206
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Authors:
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J.R.Horton,J.Zhou,X.Cheng
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Key ref:
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J.Zhou
et al.
Systematic design of adenosine analogs as inhibitors clostridioides difficile- Specific DNA adenine methyltransferase required for normal sporulation and persistence..
J med chem,
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PubMed id:
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Date:
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22-May-22
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Release date:
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11-Jan-23
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PROCHECK
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Headers
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References
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Q183J3
(Q183J3_PEPD6) -
site-specific DNA-methyltransferase (adenine-specific) from Clostridioides difficile (strain 630)
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Seq:
Struc:
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577 a.a.
545 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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T-T-C-A-A-A-A-A-G-T-C-C-C-A
14 bases
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A-T-G-G-G-A-C-T-T-T-T-T-G-A
14 bases
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T-T-C-A-A-A-A-A-G-T-C-C-C-A
14 bases
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A-T-G-G-G-A-C-T-T-T-T-T-G-A
14 bases
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T-T-C-A-A-A-A-A-G-T-C-C-C-A
14 bases
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A-T-G-G-G-A-C-T-T-T-T-T-G-A
14 bases
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Enzyme class:
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E.C.2.1.1.72
- site-specific DNA-methyltransferase (adenine-specific).
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Reaction:
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a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl- 2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H+
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2'-deoxyadenosine in DNA
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+
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S-adenosyl-L-methionine
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=
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N(6)-methyl- 2'-deoxyadenosine in DNA
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+
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S-adenosyl-L-homocysteine
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+
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H(+)
Bound ligand (Het Group name = )
matches with 51.43% similarity
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
