PDBsum entry 8clh

Cell cycle

PDB id: 8clh

Contents

Protein chains

439 a.a.

429 a.a.

123 a.a.

332 a.a.

Ligands

GTP ×2

GDP ×2

LOC

_EP ×2

POU

VLB

BKF

ACP

Metals

_CA ×2

_MG ×4

Waters ×126

PDB id:

8clh

Name:

Cell cycle

Title:

Drug cocktail (colchicine, epothilone a, peloruside, ansamitocin p3, vinblastine) bound to tubulin (t2r-ttl) complex

Structure:

Tubulin alpha-1b chain. Chain: a, c. Tubulin beta-2b chain. Chain: b, d. Stathmin-4. Chain: e. Synonym: stathmin-like protein b3,rb3. Engineered: yes. Tubulin tyrosine ligase.

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Rattus norvegicus. Norway rat. Organism_taxid: 10116. Gene: stmn4. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.

Resolution:

2.50Å R-factor: 0.185 R-free: 0.239

Authors:

M.Wranik,Q.Bertrand,M.W.Kepa,T.Weinert,M.Steinmetz,J.Standfuss

Key ref:

M.Wranik et al. (2023). A multi-Reservoir extruder for time-Resolved serial p crystallography and compound screening at x-Ray free-Electron lasers.. Nat commun, 14, 7956. PubMed id: 38042952

Date:

16-Feb-23 Release date: 13-Dec-23

Protein chains

P81947  (TBA1B_BOVIN) -  Tubulin alpha-1B chain from Bos taurus

Seq: 451 a.a.

Struc: 439 a.a.

Protein chains

Q6B856  (TBB2B_BOVIN) -  Tubulin beta-2B chain from Bos taurus

Seq: 445 a.a.

Struc: 429 a.a.

Protein chain

P63043  (STMN4_RAT) -  Stathmin-4 from Rattus norvegicus

Seq: 189 a.a.

Struc: 123 a.a.

Protein chain

E1BQ43  (E1BQ43_CHICK) -

Enzyme reactions

• Enzyme class: Chains A, C: E.C.3.6.5.- - ?????