PDBsum entry 8c4e

Cell invasion

PDB id: 8c4e

Contents

Protein chains

(+ 2 more) 370 a.a.

172 a.a.

Ligands

ADP ×8

PO4 ×8

Metals

_MG ×8

PDB id:

8c4e

Name:

Cell invasion

Title:

F-actin decorated by sipa426-685

Structure:

Actin, alpha skeletal muscle. Chain: a, b, c, d, e, f, g, h. Synonym: alpha-actin-1. Cell invasion protein sipa. Chain: k, l, m, n. Synonym: effector protein sipa. Engineered: yes

Source:

Gallus gallus. Chicken. Organism_taxid: 9031. Salmonella. Organism_taxid: 590. Gene: sipa, sspa. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Authors:

B.Yuan,J.Wald,T.C.Marlovits

Key ref:

B.Yuan et al. (2023). Structural basis for subversion of host cell actin cytoskeleton during salmonella infection.. Sci adv, 9, j5777. PubMed id: 38064550 DOI: 10.1126/SCIADV.ADJ5777

Date:

03-Jan-23 Release date: 17-Jan-24

Protein chains

P68139  (ACTS_CHICK) -  Actin, alpha skeletal muscle from Gallus gallus

Seq: 377 a.a.

Struc: 370 a.a.*

Protein chains

Q8VQB5  (SIPA_SALEN) -  Cell invasion protein SipA from Salmonella enteritidis

Seq: 685 a.a.

Struc: 172 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains A, B, C, D, E, F, G, H: E.C.3.6.4.- - ?????
• Enzyme class 3: Chains K, L, M, N: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.