PDBsum entry 8bts

Oxidoreductase

PDB id: 8bts

Contents

Protein chains

477 a.a.

522 a.a.

Ligands

HCA ×4

ICS ×4

GOL ×12

SO4 ×10

1CL ×4

Metals

_FE ×4

PDB id:

8bts

Name:

Oxidoreductase

Title:

Nitrogenase mofe protein from a. Vinelandii alpha double mutant c45a/l158c

Structure:

Nitrogenase protein alpha chain. Chain: a, c, h, j. Engineered: yes. Other_details: nifd containing two substitutions: c45a and l158c.. Nitrogenase molybdenum-iron protein beta chain. Chain: b, d, i, l. Synonym: dinitrogenase,nitrogenase component i. Engineered: yes

Source:

Azotobacter vinelandii dj. Organism_taxid: 322710. Strain: rs1. Variant: a. Vinelandii nifd: c45a/l158c. Cell_line: /. Atcc: /. Organ: /. Tissue: /. Cell: /.

Resolution:

3.03Å R-factor: 0.205 R-free: 0.223

Authors:

T.Wagner,N.Maslac

Key ref:

C.Cadoux et al. (2023). Nitrogen fixation and hydrogen evolution by stericall encumbered mo-Nitrogenase.. Jacs au, 3, 1521. PubMed id: 37234119

Date:

29-Nov-22 Release date: 07-Jun-23

Protein chains

P07328  (NIFD_AZOVI) -  Nitrogenase molybdenum-iron protein alpha chain from Azotobacter vinelandii

Seq: 492 a.a.

Struc: 477 a.a.*

Protein chains

P07329  (NIFK_AZOVI) -  Nitrogenase molybdenum-iron protein beta chain from Azotobacter vinelandii

Seq: 523 a.a.

Struc: 522 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, D, H, I, J, L: E.C.1.18.6.1 - nitrogenase.
• Pathway: Nitrogenase
• Reaction: N2 + 8 reduced [2Fe-2S]-[ferredoxin] + 16 ATP + 16 H2O = H2 + 8 oxidized [2Fe-2S]-[ferredoxin] + 2 NH4⁺ + 16 ADP + 16 phosphate + 6 H⁺
• Cofactor: Iron-sulfur; Vanadium cation or Mo cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site