PDBsum entry 8bcc

Hydrolase

PDB id: 8bcc

Contents

Protein chains

1725 a.a.

263 a.a.

Ligands

QAU ×3

EDO ×10

Waters ×422

PDB id:

8bcc

Name:

Hydrolase

Title:

Human brr2 helicase region in complex with c-tail deleted jab1 and compound 39

Structure:

U5 small nuclear ribonucleoprotein 200 kda helicase. Chain: b. Synonym: activating signal cointegrator 1 complex subunit 3-like 1, brr2 homolog,u5 snrnp-specific 200 kda protein,u5-200kd. Engineered: yes. Pre-mRNA-processing-splicing factor 8. Chain: j. Synonym: 220 kda u5 snrnp-specific protein,prp8 homolog,splicing factor prp8,p220.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: snrnp200, ascc3l1, helic2, kiaa0788. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Gene: prpf8, prpc8. Expression_system_taxid: 7111

Resolution:

2.35Å R-factor: 0.211 R-free: 0.258

Authors:

K.Vester,B.Loll,M.C.Wahl

Key ref:

K.Vester et al. Conformation-Dependent ligand hot spots in the splice RNA helicase, Brr2. To be published, . PubMed id: 36974964

Date:

15-Oct-22 Release date: 08-Mar-23

Protein chain

O75643  (U520_HUMAN) -  U5 small nuclear ribonucleoprotein 200 kDa helicase from Homo sapiens

Seq: 2136 a.a.

Struc: 1725 a.a.

Protein chain

Q6P2Q9  (PRP8_HUMAN) -  Pre-mRNA-processing-splicing factor 8 from Homo sapiens

Seq: 2335 a.a.

Struc: 263 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain B: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site