PDBsum entry 8b6t

Hydrolase

PDB id

8b6t

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Contents

			Protein chains

					517 a.a.

			Ligands

					OEH ×2

			Metals

					_CL ×2

			Waters ×308

PDB id:

8b6t

Links

Name:

Hydrolase

Title:

X-ray structure of the interface optimized haloalkane dehalogenase halotag7 fusion to the green fluorescent protein gfp (chemog5-tmr) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate

Structure:

Green fluorescent protein,haloalkane dehalogenase. Chain: a, b. Engineered: yes

Source:

Rhodococcus sp.. Organism_taxid: 1831. Gene: gfp, dhaa. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.00Å

R-factor:

0.233

R-free:

0.245

Authors:

M.Tarnawski,L.Hellweg,J.Hiblot

Key ref:

L.Hellweg et al. A general method for the development of multicolor biosensors with large dynamic ranges.. Nat chem biol, . PubMed id: 37291200

Date:

27-Sep-22

Release date:

26-Jul-23

PROCHECK

	Headers

	References

Protein chains

P0A3G3 (DHAA_RHOSO) - Haloalkane dehalogenase from Rhodococcus sp

Seq: Struc:					293 a.a. 517 a.a.*

Protein chains

P42212 (GFP_AEQVI) - Green fluorescent protein from Aequorea victoria

Seq: Struc:

Seq: Struc:					238 a.a. 517 a.a.*

Key:

PfamA domain

Secondary structure

* PDB and UniProt seqs differ at 38 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.3.8.1.5 - haloalkane dehalogenase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1-haloalkane + H2O = a halide anion + a primary alcohol + H⁺

1-haloalkane	+	H2O	=	halide anion	+	primary alcohol	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site