PDBsum entry 8b4x

Hydrolase

PDB id: 8b4x

Contents

Protein chain

473 a.a.

Ligands

3U0-ARG-TBG-LYS-OZ3

DMS ×2

Metals

_CA ×3

_NA ×4

_CL

Waters ×462

PDB id:

8b4x

Name:

Hydrolase

Title:

X-ray structure of furin (pcsk3) in complex with guanidinomethyl-phac- r-tle-k-6-(aminomethyl)-3-amino-isoindol

Structure:

Furin. Chain: a. Synonym: dibasic-processing enzyme,paired basic amino acid residue- cleaving enzyme,pace. Engineered: yes. Guanidinomethyl-phac-r-tle-k-6-(aminomethyl)-3-amino- isoindol. Chain: b. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: furin, fur, pace, pcsk3. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293s. Synthetic: yes. Synthetic construct.

Resolution:

1.60Å R-factor: 0.159 R-free: 0.170

Authors:

S.O.Dahms,H.Brandstetter

Key ref:

S.O.Dahms and h.brandstetter Aminoisoindol-Based furin inhibitors. To be published, . PubMed id: 38385828

Date:

21-Sep-22 Release date: 04-Oct-23

Protein chain

P09958  (FURIN_HUMAN) -  Furin from Homo sapiens

Seq: 794 a.a.

Struc: 473 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.21.75 - furin.
• Reaction: Release of mature proteins from their proproteins by cleavage of Arg- Xaa-Yaa-Arg-|-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors.