PDBsum entry 8aih

Transferase

PDB id: 8aih

Contents

Protein chain

184 a.a.

Ligands

SO4

2HP

Waters ×105

PDB id:

8aih

Name:

Transferase

Title:

Crystal structure of enterococcus faecium nicotinate nucleotide adenylyltransferase at 1.9 angstroms resolution

Structure:

Probable nicotinate-nucleotide adenylyltransferase. Chain: a. Synonym: deamido-NAD(+) diphosphorylase,deamido-NAD(+) pyrophosphorylase,nicotinate mononucleotide adenylyltransferase,namn adenylyltransferase. Engineered: yes

Source:

Enterococcus faecium. Organism_taxid: 1352. Gene: nadd, nadd_1, b1p95_04405, bu194_05695, dtpha_1401727, hmpref3199_01612. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.90Å R-factor: 0.238 R-free: 0.279

Authors:

R.Pandian,O.A.Jeje,Y.Sayed,I.A.Achilonu

Key ref:

O.Jeje et al. (2023). Obtaining high yield recombinant enterococcus faecium nicotinate nucleotide adenylyltransferase for X-Ray crystallography and biophysical studies.. Int.J.Biol.Macromol., 250, 26066. PubMed id: 37544558 DOI: 10.1016/J.IJBIOMAC.2023.126066

Date:

26-Jul-22 Release date: 16-Aug-23

Protein chain

A0A133MWI0  () -  Probable nicotinate-nucleotide adenylyltransferase from Enterococcus faecium

Seq: 216 a.a.

Struc: 184 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.7.18 - nicotinate-nucleotide adenylyltransferase.
• Reaction: nicotinate beta-D-ribonucleotide + ATP + H⁺ = deamido-NAD⁺ + diphosphate

nicotinate beta-D-ribonucleotide + ATP + H(+) = deamido-NAD(+) + diphosphate (Bound ligand (Het Group name = 2HP) matches with 55.56% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site