PDBsum entry 8af7

Hydrolase

PDB id: 8af7

Contents

Protein chain

261 a.a.

Ligands

SO4

Waters ×174

PDB id:

8af7

Name:

Hydrolase

Title:

Room temperature ssx crystal structure of ctx-m-14 (10k dataset)

Structure:

Beta-lactamase. Chain: a. Engineered: yes

Source:

Klebsiella pneumoniae. Organism_taxid: 573. Gene: ctx-m-14. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.55Å R-factor: 0.181 R-free: 0.215

Authors:

D.Oberthuer,M.Perbandt,A.Prester,H.Rohde,C.Betzel,O.Yefanov

Key ref:

K.A.Zielinski et al. (2022). Rapid and efficient room-Temperature serial synchrotr crystallography using the cfel tapedrive. Iucrj, 9, 778. PubMed id: 36381150

Date:

15-Jul-22 Release date: 16-Nov-22

Protein chain

A0A0H3H219  (A0A0H3H219_KLEPH) -  Beta-lactamase from Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)

Seq: 291 a.a.

Struc: 261 a.a.

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)