PDBsum entry 8a8f

Gene regulation

PDB id: 8a8f

Contents

Protein chains

295 a.a.

59 a.a.

Ligands

PO4

Metals

_MN ×2

Waters ×121

PDB id:

8a8f

Name:

Gene regulation

Title:

Crystal structure of glc7 phosphatase in complex with the regulatory region of ref2

Structure:

Serine/threonine-protein phosphatase pp1-2. Chain: a. Engineered: yes. Other_details: the glc7 c-terminus is covalently linked to chain b (ref2 348-405). RNA end formation protein 2. Chain: b. Engineered: yes. Other_details: to trap the glc7 substrate in the active site, two RNA

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Strain: atcc 204508 / s288c. Gene: glc7, cid1, dis2, yer133w. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768. Gene: ref2, ydr195w, yd9346.06. Expression_system_taxid: 866768

Resolution:

1.85Å R-factor: 0.194 R-free: 0.221

Authors:

M.Carminati,C.M.Manav,D.Bellini,L.A.Passmore

Key ref:

M.Carminati et al. Crystal structure of glc7 phosphatase in complex with regulatory region of ref2. To be published, . PubMed id: 38029752

Date:

22-Jun-22 Release date: 29-Nov-23

Protein chain

P32598  (PP12_YEAST) -  Serine/threonine-protein phosphatase PP1-2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 312 a.a.

Struc: 295 a.a.

Protein chain

P42073  (REF2_YEAST) -  RNA end formation protein 2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 533 a.a.

Struc: 59 a.a.

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate

O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

• Enzyme class 3: Chain B: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site