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PDBsum entry 8a7h
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protein ligands Protein-protein interface(s) links
Signaling protein PDB id
8a7h

 

 

 

 

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Contents
Protein chains
362 a.a.
Ligands
ATP ×2
JGC ×2
PDB id:
8a7h
Name: Signaling protein
Title: Crystal structure of a chimeric lov-histidine kinase sb2f1-i66r mutant (light state; asymmetrical variant, trigonal form with long c axis)
Structure: Putative sensory box protein,sensor protein fixl. Chain: a, b. Engineered: yes. Mutation: yes. Other_details: his-tag: 1-mgsshhhhhhssglvprgsh-20. Belongs to q88jb0: 21 (or 1 PDB-file)-mina...Yyigiqrdvt-140 (or 120). Belongs to p23222:141 (or 121 PDB-file)-ehqqtq...Aaden-388 (or 368). Unp:q88jb0 (unp-numbering) 4-mina...Qrdvt-123. Unp:p23222 (unp-numbering) 258 to c-term. Engineered mutation: ile 66 arg.
Source: Pseudomonas putida kt2440, bradyrhizobium diazoefficiens (strain jcm 10833 / bcrc 13528 / iam 13628 / nbrc 14792 / usda 110). Organism_taxid: 160488, 224911. Strain: atcc 47054 / dsm 6125 / cfbp 8728 / ncimb 11950 / kt2440, jcm 10833 / bcrc 13528 / iam 13628 / nbrc 14792 / usda 110. Gene: pp_2739, fixl, bll2760. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768
Resolution:
3.15Å     R-factor:   0.257     R-free:   0.306
Authors: V.Arinkin,J.Granzin,R.Batra-Safferling
Key ref: V.Arinkin et al. Crystal structure of a chimeric lov-Histidine kinase sb2f1-I66r mutant (light state. To be published, .
Date:
21-Jun-22     Release date:   10-Jan-24    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q88JB0  (Q88JB0_PSEPK) -  Sensory box protein from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Seq:
Struc: 		151 a.a.
362 a.a.*
Protein chains
Pfam   ArchSchema ?
P23222  (FIXL_BRADU) -  Sensor protein FixL from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)
Seq:
Struc: 		505 a.a.
362 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 117 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.2.7.13.3  - histidine kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
ATP
Bound ligand (Het Group name = ATP)
corresponds exactly 		+ protein L-histidine
 = ADP
 + protein N-phospho-L-histidine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

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