PDBsum entry 7zr5

Protein binding

PDB id: 7zr5

Contents

Protein chains

633 a.a.

266 a.a.

186 a.a.

429 a.a.

Ligands

ATP ×2

PDB id:

7zr5

Name:

Protein binding

Title:

Cryoem structure of hsp90-cdc37-braf(v600e)-pp5(closed) complex

Structure:

Heat shock protein hsp 90-beta. Chain: a, b. Synonym: hsp 90,heat shock 84 kda,hsp 84,hsp84. Engineered: yes. Hsp90 co-chaperone cdc37. Chain: c. Synonym: hsp90 chaperone protein kinase-targeting subunit,p50cdc37. Engineered: yes. Serine/threonine-protein kinase b-raf.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hsp90ab1, hsp90b, hspc2, hspcb. Expressed in: spodoptera. Expression_system_taxid: 7106. Gene: cdc37, cdc37a. Gene: braf, braf1, rafb1. Gene: ppp5c, ppp5.

Authors:

J.Oberoi,L.H.Pearl

Key ref:

J.Oberoi et al. (2022). Hsp90-Cdc37-Pp5 forms a structural platform for kinas dephosphorylation.. Nat commun, 13, 7343. PubMed id: 36446791

Date:

03-May-22 Release date: 14-Dec-22

Protein chains

P08238  (HS90B_HUMAN) -  Heat shock protein HSP 90-beta from Homo sapiens

Seq: 724 a.a.

Struc: 633 a.a.

Protein chain

Q16543  (CDC37_HUMAN) -  Hsp90 co-chaperone Cdc37 from Homo sapiens

Seq: 378 a.a.

Struc: 266 a.a.

Protein chain

P15056  (BRAF_HUMAN) -  Serine/threonine-protein kinase B-raf from Homo sapiens

Seq: 766 a.a.

Struc: 186 a.a.

Protein chain

P53041  (PPP5_HUMAN) -  Serine/threonine-protein phosphatase 5 from Homo sapiens

Seq: 499 a.a.

Struc: 429 a.a.

Enzyme reactions

• Enzyme class 1: Chain K: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP (Bound ligand (Het Group name = ATP) corresponds exactly) = O-phospho-L-seryl-[protein] + ADP + H(+)

L-threonyl-[protein] + ATP (Bound ligand (Het Group name = ATP) corresponds exactly) = O-phospho-L-threonyl-[protein] + ADP + H(+)

• Enzyme class 2: Chain P: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site