PDBsum entry 7z8b

Ligase

PDB id: 7z8b

Contents

Protein chains

1224 a.a.

457 a.a.

86 a.a.

129 a.a.

Metals

_ZN ×3

PDB id:

7z8b

Name:

Ligase

Title:

Structure of crl7fbxw8 reveals coupling with cul1-rbx1/roc1 for multi- cullin-ring e3-catalyzed ubiquitin ligation

Structure:

Cullin-7. Chain: c. Synonym: cul-7. Engineered: yes. F-box/wd repeat-containing protein 8. Chain: f. Synonym: f-box and wd-40 domain-containing protein 8,f-box only protein 29. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cul7, kiaa0076. Expressed in: homo sapiens. Expression_system_taxid: 9606. Gene: fbxw8, fbw6, fbw8, fbx29, fbxo29, fbxw6. Gene: rbx1, rnf75, roc1. Gene: skp1, emc19, ocp2, skp1a, tceb1l.

Authors:

L.V.M.Hopf,B.A.Schulman

Key ref:

L.V.M.Hopf et al. Structure of crl7fbxw8 reveals coupling with cul1-Rbx for multi-Cullin-Ring e3-Catalyzed ubiquitin ligation. Nat struct mol biol, . PubMed id: 35982156

Date:

17-Mar-22 Release date: 24-Aug-22

Protein chain

Q14999  (CUL7_HUMAN) -  Cullin-7 from Homo sapiens

Seq: 1698 a.a.

Struc: 1224 a.a.*

Protein chain

Q8N3Y1  (FBXW8_HUMAN) -  F-box/WD repeat-containing protein 8 from Homo sapiens

Seq: 598 a.a.

Struc: 457 a.a.

Protein chain

P62877  (RBX1_HUMAN) -  E3 ubiquitin-protein ligase RBX1 from Homo sapiens

Seq: 108 a.a.

Struc: 86 a.a.

Protein chain

P63208  (SKP1_HUMAN) -  S-phase kinase-associated protein 1 from Homo sapiens

Seq: 163 a.a.

Struc: 129 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chain R: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 3: Chain R: E.C.2.3.2.32 - cullin-RING-type E3 NEDD8 transferase.
• Reaction: S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]- L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N₆-[NEDD8- protein]-yl-[cullin]-L-lysine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.