PDBsum entry 7z6h

DNA binding protein

PDB id: 7z6h

Contents

Protein chains

247 a.a.

260 a.a.

273 a.a.

324 a.a.

338 a.a.

315 a.a.

317 a.a.

439 a.a.

DNA/RNA

Ligands

AGS ×5

PDB id:

7z6h

Name:

DNA binding protein

Title:

Structure of DNA-bound human rad17-rfc clamp loader and 9-1-1 checkpoint clamp

Structure:

Cell cycle checkpoint control protein rad9a. Chain: a. Synonym: hrad9,DNA repair exonuclease rad9 homolog a. Engineered: yes. Cell cycle checkpoint protein rad1,cell cycle checkpoint protein rad17. Chain: b, k. Synonym: hrad1,DNA repair exonuclease rad1 homolog,rad1-like DNA damage checkpoint protein,hrad17,rf-c/activator 1 homolog.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: rad9a. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: rad1, rec1, rad17, r24l. Gene: hus1. Gene: rfc4.

Authors:

M.Day,A.W.Oliver,L.H.Pearl

Key ref:

M.Day et al. Structure of DNA-Bound human rad17-Rfc clamp loader a 9-1-1 checkpoint clamp. To be published, . PubMed id: 35819203

Date:

11-Mar-22 Release date: 04-May-22

Protein chain

Q99638  (RAD9A_HUMAN) -  Cell cycle checkpoint control protein RAD9A from Homo sapiens

Seq: 391 a.a.

Struc: 247 a.a.

Protein chain

O60671  (RAD1_HUMAN) -  Cell cycle checkpoint protein RAD1 from Homo sapiens

Seq: 282 a.a.

Struc: 260 a.a.

Protein chain

O75943  (RAD17_HUMAN) -  Cell cycle checkpoint protein RAD17 from Homo sapiens

Seq: 681 a.a.

Struc: 260 a.a.*

Protein chain

O60921  (HUS1_HUMAN) -  Checkpoint protein HUS1 from Homo sapiens

Seq: 280 a.a.

Struc: 273 a.a.

Protein chain

P35249  (RFC4_HUMAN) -  Replication factor C subunit 4 from Homo sapiens

Seq: 363 a.a.

Struc: 324 a.a.

Protein chain

P40938  (RFC3_HUMAN) -  Replication factor C subunit 3 from Homo sapiens

Seq: 356 a.a.

Struc: 338 a.a.

Protein chain

P35250  (RFC2_HUMAN) -  Replication factor C subunit 2 from Homo sapiens

Seq: 354 a.a.

Struc: 315 a.a.

Protein chain

P40937  (RFC5_HUMAN) -  Replication factor C subunit 5 from Homo sapiens

Seq: 340 a.a.

Struc: 317 a.a.

Protein chain

O60671  (RAD1_HUMAN) -  Cell cycle checkpoint protein RAD1 from Homo sapiens

Seq: 282 a.a.

Struc: 439 a.a.*

Protein chain

O75943  (RAD17_HUMAN) -  Cell cycle checkpoint protein RAD17 from Homo sapiens

Seq: 681 a.a.

Struc: 439 a.a.

* PDB and UniProt seqs differ at 418 residue positions (black crosses)

DNA/RNA chains

A-T-A-T-A-C-G-G-G-C-T-G-C-T-G-T-T-G-A-C-A-A 22 bases

C-C-C-G-T-A-T-A-T-T 10 bases

Enzyme reactions

• Enzyme class: Chains A, B, K: E.C.3.1.11.2 - exodeoxyribonuclease Iii.
• Reaction: Degradation of double-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.