PDBsum entry 7xwh

Oxidoreductase

PDB id: 7xwh

Contents

Protein chains

223 a.a.

Ligands

NAP ×4

Waters ×628

PDB id:

7xwh

Name:

Oxidoreductase

Title:

Structure of patulin-detoxifying enzyme with NADP+

Structure:

Short-chain dehydrogenase/reductase. Chain: a, b, c, d. Engineered: yes

Source:

Meyerozyma guilliermondii. Organism_taxid: 4929. Gene: sdr. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.31Å R-factor: 0.190 R-free: 0.272

Authors:

L.Dai,H.Li,Y.Hu,R.T.Guo,C.C.Chen

Key ref:

L.Dai et al. (2022). Structure-Based rational design of a short-Chain dehydrogenase/reductase for improving activity toward mycotoxin patulin.. Int.J.Biol.Macromol., 222, 421. PubMed id: 36176222 DOI: 10.1016/J.IJBIOMAC.2022.09.121

Date:

26-May-22 Release date: 26-Oct-22

Protein chains

A0A888VSF1  (A0A888VSF1_PICGM) -  Short-chain dehydrogenase/reductase from Meyerozyma guilliermondii

Seq: 250 a.a.

Struc: 223 a.a.

Enzyme reactions

• Enzyme class: E.C.1.1.1.1 - alcohol dehydrogenase.
• Reaction:
  1. a primary alcohol + NAD⁺ = an aldehyde + NADH + H⁺
  2. a secondary alcohol + NAD⁺ = a ketone + NADH + H⁺

primary alcohol + NAD(+) (Bound ligand (Het Group name = NAP) matches with 91.67% similarity) = aldehyde + NADH + H(+)

secondary alcohol + NAD(+) (Bound ligand (Het Group name = NAP) matches with 91.67% similarity) = ketone + NADH + H(+)

• Cofactor: Zn(2+) or Fe cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site