PDBsum entry 7x7h

Gene regulation/transferase

PDB id: 7x7h

Contents

Protein chains

267 a.a.

85 a.a.

Metals

_CA ×2

Waters ×446

PDB id:

7x7h

Name:

Gene regulation/transferase

Title:

Crystal structure of fructose regulator/histidine phosphocarrier protein complex from vibrio cholerae

Structure:

Catabolite repressor/activator. Chain: a, c. Engineered: yes. Hpr family phosphocarrier protein. Chain: b, d. Synonym: pts sugar transporter,phosphocarrier protein hpr. Engineered: yes

Source:

Vibrio cholerae. Organism_taxid: 666. Gene: cra, d6u24_17570, eyb64_06550, flm02_11955, fxe67_10910, hpy07_16365. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ptsh, bc353_05360, d6u24_08050, ers013138_01023, ers013165_01775, ers013166_02939, ers013186_02269, ers013193_00498, ers013198_00582, ers013199_02080, ers013200_03874, ers013201_03670,

Resolution:

2.00Å R-factor: 0.181 R-free: 0.227

Authors:

M.-K.Kim,J.Zhang,C.-K.Yoon,Y.-J.Seok

Key ref:

M.-K.Kim et al. Crystal structure of fructose regulator/histidine phosphocarrier protein complex from vibrio cholerae. To be published, . PubMed id: 36987873

Date:

09-Mar-22 Release date: 15-Mar-23

Protein chains

Q9KM69  (Q9KM69_VIBCH) -  Fructose operon regulatory protein from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

Seq: 326 a.a.

Struc: 267 a.a.

Protein chains

Q9KTD6  (PTHP_VIBCH) -  Phosphocarrier protein HPr from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

Seq: 85 a.a.

Struc: 85 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains A, C: E.C.?
• Enzyme class 3: Chains B, D: E.C.2.7.11.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.