PDBsum entry 7wpl

Ligase

PDB id: 7wpl

Contents

Protein chain

510 a.a.

Ligands

ATP

EDO ×2

Waters ×32

PDB id:

7wpl

Name:

Ligase

Title:

Methionyl-tRNA synthetase from staphylococcus aureus complexed with atp

Structure:

Methionine--tRNA ligase. Chain: a. Synonym: methionyl-tRNA synthetase,metrs. Engineered: yes

Source:

Staphylococcus aureus subsp. Aureus col. Staphylococcus aureus col. Organism_taxid: 93062. Strain: col. Gene: metg. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.50Å R-factor: 0.226 R-free: 0.271

Authors:

J.Yi,Z.Cai,H.Qiu,F.Lu,B.Chen,Z.Luo,Q.Gu,J.Xu,H.Zhou

Key ref:

J.Yi et al. Fragment screening and structural analyses highlight ATP-Assisted ligand binding for inhibitor discovery a type 1 methionyl-Trna synthetase. Nucleic acids res, . PubMed id: 35474479

Date:

24-Jan-22 Release date: 27-Apr-22

Protein chain

Q5HII6  (SYM_STAAC) -  Methionine--tRNA ligase from Staphylococcus aureus (strain COL)

Seq: 657 a.a.

Struc: 510 a.a.

Enzyme reactions

• Enzyme class: E.C.6.1.1.10 - methionine--tRNA ligase.
• Reaction: tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate

tRNA(Met) (Bound ligand (Het Group name = ATP) corresponds exactly) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site