PDBsum entry 7v0d

Oxidoreductase

PDB id: 7v0d

Contents

Protein chains

527 a.a.

Ligands

GOL ×5

1PE

PEG ×2

SO4 ×7

EPE

Metals

_CL

Waters ×323

PDB id:

7v0d

Name:

Oxidoreductase

Title:

Crystal structure of halogenase ctcp from kitasatospora aureofaciens

Structure:

Tetracycline 7-halogenase. Chain: a, b. Synonym: fadh2-dependent halogenase. Engineered: yes. Other_details: the n-terminal gph is a cloning artifact left after n- terminal tag cleavage

Source:

Kitasatospora aureofaciens. Organism_taxid: 1894. Gene: ctcp, cts4, b6264_18525, hs99_0013305. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.60Å R-factor: 0.188 R-free: 0.219

Authors:

C.Hou,O.V.Tsodikov

Key ref:

C.Hou et al. Crystal structures and complex formation of halogenas and FAD reductase ctcq from the chlortetracycline biosynthetic pathway. To be published, .

Date:

10-May-22 Release date: 17-May-23

Protein chains

A0A1E7MYN1  (CTCP_KITAU) -  Tetracycline 7-halogenase from Kitasatospora aureofaciens

Seq: 555 a.a.

Struc: 527 a.a.

Enzyme reactions

• Enzyme class: E.C.1.14.19.49 - tetracycline 7-halogenase.
• Reaction: tetracycline + FADH2 + chloride + O2 = 7-chlorotetracycline + FAD + 2 H2O + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site