PDBsum entry 7v0b

Oxidoreductase

PDB id: 7v0b

Contents

Protein chains

539 a.a.

Ligands

FAD ×4

GOL ×3

EPE ×2

Metals

_CL ×4

_NA

Waters ×942

PDB id:

7v0b

Name:

Oxidoreductase

Title:

Crystal structure of halogenase ctcp from kitasatospora aureofaciens in complex with fad

Structure:

Tetracycline 7-halogenase. Chain: a, b, c, d. Synonym: fadh2-dependent halogenase. Engineered: yes. Other_details: the n-terminal gph sequence is a cloning artifact, left from the n-terminal tag after cleavage.

Source:

Kitasatospora aureofaciens. Organism_taxid: 1894. Gene: ctcp, cts4, b6264_18525, hs99_0013305. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.15Å R-factor: 0.190 R-free: 0.233

Authors:

C.Hou,O.V.Tsodikov

Key ref:

C.Hou et al. Crystal structures and complex formation of halogenas and FAD reductase ctcq from the chlortetracycline biosynthetic pathway. To be published, .

Date:

10-May-22 Release date: 17-May-23

Protein chains

A0A1E7MYN1  (CTCP_KITAU) -  Tetracycline 7-halogenase from Kitasatospora aureofaciens

Seq: 555 a.a.

Struc: 539 a.a.

Enzyme reactions

• Enzyme class: E.C.1.14.19.49 - tetracycline 7-halogenase.
• Reaction: tetracycline + FADH2 + chloride + O2 = 7-chlorotetracycline + FAD + 2 H2O + H⁺

tetracycline + FADH2 (Bound ligand (Het Group name = FAD) corresponds exactly) + chloride + O2 = 7-chlorotetracycline + FAD + 2 × H2O + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site