PDBsum entry 7uvc

Transferase/ligase

PDB id: 7uvc

Contents

Protein chains

149 a.a.

160 a.a.

PDB id:

7uvc

Name:

Transferase/ligase

Title:

Rad6(p43l)-bre1 complex

Structure:

Ubiquitin-conjugating enzyme e2 2. Chain: a. Synonym: e2 ubiquitin-conjugating enzyme 2,radiation sensitivity protein 6,ubiquitin carrier protein ubc2,ubiquitin-protein ligase ubc2. Engineered: yes. Mutation: yes. E3 ubiquitin-protein ligase bre1. Chain: v, u.

Source:

Saccharomyces cerevisiae s288c. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: rad6, ubc2, ygl058w. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: bre1, ydl074c.

Resolution:

3.05Å R-factor: 0.237 R-free: 0.279

Authors:

P.K.Shukla,M.B.Chandrasekharan

Key ref:

P.K.Shukla and m.b.chandrasekharan Rad6(p43l)-Bre1 complex. To be published, . PubMed id: 36715322

Date:

29-Apr-22 Release date: 11-Jan-23

Protein chain

P06104  (UBC2_YEAST) -  Ubiquitin-conjugating enzyme E2 2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 172 a.a.

Struc: 149 a.a.*

Protein chains

Q07457  (BRE1_YEAST) -  E3 ubiquitin-protein ligase BRE1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 700 a.a.

Struc: 160 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.2.3.2.23 - E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine
• Enzyme class 3: Chains V, U: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.