PDBsum entry 7ut9

Oxidoreductase

PDB id: 7ut9

Contents

Protein chains

477 a.a.

522 a.a.

271 a.a.

Ligands

HCA ×2

ICS ×2

CLF ×2

ADP

SF4

ATP

Metals

_FE ×2

_MG ×2

Waters ×60

PDB id:

7ut9

Name:

Oxidoreductase

Title:

Cryoem structure of azotobacter vinelandii nitrogenase complex (1:1 fep:mofep, adp/atp-bound) during catalytic n2 reduction

Structure:

Nitrogenase molybdenum-iron protein alpha chain. Chain: a, c. Synonym: dinitrogenase,nitrogenase component i. Nitrogenase molybdenum-iron protein beta chain. Chain: b, d. Synonym: dinitrogenase. Nitrogenase iron protein gamma chain. Chain: e, f. Synonym: nitrogenase fe protein,nitrogenase component ii,nitrogenase

Source:

Azotobacter vinelandii dj. Organism_taxid: 322710. Strain: dj / atcc baa-1303

Authors:

H.L.Rutledge,B.Cook,F.A.Tezcan,M.A.Herzik

Key ref:

H.L.Rutledge et al. Structures of the nitrogenase complex prepared under catalytic turnover conditions.. Science, . PubMed id: 35901182

Date:

26-Apr-22 Release date: 17-Aug-22

Protein chains

P07328  (NIFD_AZOVI) -  Nitrogenase molybdenum-iron protein alpha chain from Azotobacter vinelandii

Seq: 492 a.a.

Struc: 477 a.a.

Protein chains

P07329  (NIFK_AZOVI) -  Nitrogenase molybdenum-iron protein beta chain from Azotobacter vinelandii

Seq: 523 a.a.

Struc: 522 a.a.

Protein chains

P00459  (NIFH1_AZOVI) -  Nitrogenase iron protein 1 from Azotobacter vinelandii

Seq: 290 a.a.

Struc: 271 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.1.18.6.1 - nitrogenase.
• Pathway: Nitrogenase
• Reaction: N2 + 8 reduced [2Fe-2S]-[ferredoxin] + 16 ATP + 16 H2O = H2 + 8 oxidized [2Fe-2S]-[ferredoxin] + 2 NH4⁺ + 16 ADP + 16 phosphate + 6 H⁺

N2 (Bound ligand (Het Group name = ATP) corresponds exactly) + 8 × reduced [2Fe-2S]-[ferredoxin] + 16 × ATP + 16 × H2O = H2 (Bound ligand (Het Group name = ADP) corresponds exactly) + 8 × oxidized [2Fe-2S]-[ferredoxin] + 2 × NH4(+) + 16 × ADP + 16 × phosphate + 6 × H(+)

• Cofactor: Iron-sulfur; Vanadium cation or Mo cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site